Proteintech, 30358-1-AP, FGFR1 Polyclonal antibody

Size: 20ul / 150ul The FGFR1 (30358-1-AP) by Proteintech is a Polyclonal antibody targeting FGFR1 in IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 30358-1-AP targets FGFR1 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive IP detected in: SH-SY5Y cells Positive IHC detected in: human colon tissue, mouse brain tissue, rat kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: SH-SY5Y cells Recommended dilution Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Fibroblast growth factor receptor 1 (FGFR1) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors. FGFR1 has been implicated in diverse biological processes including embryonic development, cell proliferation, differentiation, migration, and tumorigenesis. Mutations in the FGFR1 gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer, and autosomal dominant Kallmann syndrome. Alternatively spliced variants that encode different protein isoforms have been described. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: fibroblast growth factor receptor 1 Calculated Molecular Weight: 820 aa, 91 kDa Observed Molecular Weight: 80 kDa, 95 kDa, 105 kDa GenBank Accession Number: BC018128 Gene Symbol: FGFR1 Gene ID (NCBI): 2260 RRID: AB_3669716 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P11362 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.