Proteintech, 30373-1-AP, RB1 Polyclonal antibody

Size: 20ul / 150ul The RB1 (30373-1-AP) by Proteintech is a Polyclonal antibody targeting RB1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 30373-1-AP targets RB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: K-562 cells, HT-1080 cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information The RB1 gene, a member of the pocket family with p107 and p130, was the first known tumor suppressor. RB1protein is targeted by the transforming proteins of the DNA tumor viruses like adenoviral E1A and is functionally inactivated in most human tumor cells owing to mutations of either the RB1 gene itself or its upstream regulators (PMID: 22787429). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: retinoblastoma 1 Calculated Molecular Weight: 928 aa, 106 kDa Observed Molecular Weight: 110 kDa GenBank Accession Number: BC039060 Gene Symbol: RB1 Gene ID (NCBI): 5925 RRID: AB_3086301 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P06400 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.