Proteintech, 30437-1-AP, Periphilin 1 Polyclonal antibody

Size: 20ul / 150ul The Periphilin 1 (30437-1-AP) by Proteintech is a Polyclonal antibody targeting Periphilin 1 in WB, IHC, ELISA applications with reactivity to human, mouse samples 30437-1-AP targets Periphilin 1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A549 cells, Calu-1 cells, Jurkat cells, MG-63 cells, U2OS cells Positive IHC detected in: mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Periphilin 1 is a component of the HUSH complex, which is a multiprotein complex that mediates epigenetic repression. The HUSH complex is recruited to genomic loci rich in H3K9me3 and is likely necessary for maintaining transcriptional silencing by promoting the recruitment of SETDB1, a histone methyltransferase that mediates the deposition of additional H3K9me3. Periphilin 1 contributes to the maintenance of the HUSH complex at chromatin (PMID: 26022416). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag32768 Product name: Recombinant human PPHLN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 33-200 aa of BC039832 Sequence: ESGYRWTRDDHSASRQPEYRDMRDGFRRKSFYSSHYARERSPYKRDNTFFRESPVGRKDSPHSRSGSSVSSRSYSPERSKSYSFHQSQHRKSVRPGASYKRQNEGNPERDKERPVQSLKTSRDTSPSSGSAVSSSKVLDKPSRLTEKELAEAASKWAAEKLEKSDESN Predict reactive species Full Name: periphilin 1 Calculated Molecular Weight: 458 aa, 53 kDa Observed Molecular Weight: 37-70 kDa GenBank Accession Number: BC039832 Gene Symbol: PPHLN1 Gene ID (NCBI): 51535 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8NEY8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.