Proteintech, 30537-1-AP, IL-12/IL-23 p40 Polyclonal antibody

Size: 20ul / 150ul The IL-12/IL-23 p40 (30537-1-AP) by Proteintech is a Polyclonal antibody targeting IL-12/IL-23 p40 in WB, ELISA applications with reactivity to mouse, rat samples 30537-1-AP targets IL-12/IL-23 p40 in WB, ELISA applications and shows reactivity with mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, mouse heart tissue, mouse kidney tissue, rat brain tissue, rat heart tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information Interleukin (IL)-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918). Specification Tested Reactivity: mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: interleukin 12b Calculated Molecular Weight: 38kd Observed Molecular Weight: 45 kDa GenBank Accession Number: NM_001303244 Gene Symbol: Il12b Gene ID (NCBI): 16160 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P43432 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.