Proteintech, 30697-1-AP, ABCC10 Polyclonal antibody

Size: 20ul / 150ul The ABCC10 (30697-1-AP) by Proteintech is a Polyclonal antibody targeting ABCC10 in WB, IHC, ELISA applications with reactivity to human, mouse samples 30697-1-AP targets ABCC10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293T cells, HeLa cells Positive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information The ATP binding cassette sub-family C member 10 (ABCC10), also named MRP7, is a cellular transmembrane protein transporter and belongs to the ATP-binding cassette (ABC) family. MRP7 is a lipophilic anion pump responsible for the transport of glucuronide E(2)17betaG and can confer resistance to chemotherapy drugs (PMID: 16868766). Since it is glycosylated, the apparent molecular weight of ABCC10 could be variable, ranging from 161 kDa to 191 kDa (PMID: 23087055; PMID: 24103790; PMID: 31338999; PMID: 34631561). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag33719 Product name: Recombinant human ABCC10 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1203-1492 aa of NM_001198934.1 Sequence: SSFTQTEAMLVSVERLEEYTCDLPQEPQGQPLQLGTGWLTQGGVEFQDVVLAYRPGLPNALDGVTFCVQPGEKLGIVGRTGSGKSSLLLVLFRLLEPSSGRVLLDGVDTSQLELAQLRSQLAIIPQEPFLFSGTVRENLDPQGLHKDRALWQALKQCHLSEVITSMGGLDGELGEGGRSLSLGQRQLLCLARALLTDAKILCIDEATASVDQKTDQLLQQTICKRFANKTVLTIAHRLNTILNSDRVLVLQAGRVVELDSPATLRNQPHSLFQQLLQSSQQGVPASLGGP* Predict reactive species Full Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 10 Calculated Molecular Weight: 161kd Observed Molecular Weight: ~190 kDa GenBank Accession Number: NM_001198934.1 Gene Symbol: ABCC10 Gene ID (NCBI): 89845 RRID: AB_3086392 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q5T3U5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.