Proteintech, 30721-1-AP, COA4 Polyclonal antibody

Size: 20ul / 150ul The COA4 (30721-1-AP) by Proteintech is a Polyclonal antibody targeting COA4 in WB, IHC, ELISA applications with reactivity to human samples 30721-1-AP targets COA4 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: LNCaP cells, HEK-293 cells, HeLa cells, PC-3 cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information The coiled-coil-helix-coiled-coil-helix domain containing 8 (CHCHD8) is a putative COX assembly factor known as cytochrome c oxidase assembly factor 4 homolog (CoA4). COA4 is a newly identified CcO assembly factor which is a twin CX(9)C motif mitochondrial protein localized in the intermembrane space linked with the inner membrane of mitochondria. Its transport into intermembrane space depends on the MIA40 trans-site receptor machinery. (PMID: 37345060) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag33966 Product name: Recombinant human CHCHD8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-87 aa of NM_016565.2 Sequence: MSTSVPQGHTWTQRVKKDDEEEDPLDQLISRSGCAASHFAVQECMAQHQDWRQCQPQVQAFKDCMSEQQARRQEELQRRQEQAGAHH Predict reactive species Full Name: Cytochrome c oxidase assembly factor 4 homolog, mitochondrial Calculated Molecular Weight: 10 kDa Observed Molecular Weight: 12 kDa GenBank Accession Number: NM_016565.2 Gene Symbol: COA4 Gene ID (NCBI): 51287 RRID: AB_3669754 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NYJ1-1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.