Proteintech, 30867-1-AP, SGOL2 Polyclonal antibody

Size: 20ul / 150ul The SGOL2 (30867-1-AP) by Proteintech is a Polyclonal antibody targeting SGOL2 in WB, IHC, IF/ICC, ELISA applications with reactivity to Human, mouse samples 30867-1-AP targets SGOL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples. Tested Applications Positive WB detected in: HT-1080 cells, HeLa cells, U2OS cells Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information SGOL2 (Shugoshin 2, also known as SGO2), which is tightly involved in the cell cycle process, has been reported to protect cohesion, sustain the linkage of chromosomes, and regulate kinetochore-microtubule attachment in meiosis and mitosis (PMID: 21273764). SGOL2 has been reported as a differentially expressed gene in various types of cancer, including glioma, hepatocellular cancer, and endometrial cancer (PMID: 36566018). Specification Tested Reactivity: Human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag29656 Product name: Recombinant human SGOL2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-232 aa of BC092412 Sequence: MECPVMETDSLFTSGIKRHLKDKRISKTTKLNVSLASKIKTKILNNSSIFKISLKHNNRALAQALSREKENSRRITTEKMLLQKEVEKLNFENTFLRLKLNNLNKKLIDIEALMNNNLITAIEMSSLSEFHQSSFLLSASKKKRISKQCKLMRLPFARVPLTSNDDEDEDKEKMQCDNNIKSKTLPDIPSSGSTTQPLSTQDNSEVLFLKENNQNVYGLDDSEHISSIVDVP Predict reactive species Full Name: shugoshin-like 2 (S. pombe) Calculated Molecular Weight: 1265 aa, 145 kDa Observed Molecular Weight: 180 kDa GenBank Accession Number: BC092412 Gene Symbol: SGOL2 Gene ID (NCBI): 151246 RRID: AB_3669770 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q562F6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.