Proteintech, 30914-1-AP, SAGE1 Polyclonal antibody

Size: 20ul / 150ul The SAGE1 (30914-1-AP) by Proteintech is a Polyclonal antibody targeting SAGE1 in IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 30914-1-AP targets SAGE1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive IHC detected in: human skin cancer tissue, mouse testis tissue, rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A375 cells, U2OS cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:100-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Sarcoma antigen 1 (SAGE1) is a cancer/testis antigen that is limited to the spermatogonia and pre-meiotic spermatocytes and localized to the nuclei (PMID: 34465596). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag34216 Product name: Recombinant human SAGE1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 247-387 aa of NM_018666.2 Sequence: ATITYNVPEEKMEKGQPQPDNILSTASTGLINVAGAGTPAISTNGLYSTVPHNVCEEKMENDQPQPNNVLSTVQPVIIYLTATGIPGMNTRDQYATITHNVCEERVVNNQPLPSNALSTVLPGLAYLATADMPAMSTRDQH Predict reactive species Full Name: sarcoma antigen 1 Calculated Molecular Weight: 99kd GenBank Accession Number: NM_018666.2 Gene Symbol: SAGE1 Gene ID (NCBI): 55511 RRID: AB_3669783 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q9NXZ1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.