Proteintech, 31120-1-AP, CD34 Polyclonal antibody

Size: 20ul / 150ul The CD34 (31120-1-AP) by Proteintech is a Polyclonal antibody targeting CD34 in IHC, ELISA applications with reactivity to Mouse samples 31120-1-AP targets CD34 in IHC, IF, ELISA applications and shows reactivity with Mouse samples. Tested Applications Positive IHC detected in: mouse spleen tissue, mouse kidney tissue, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. Specification Tested Reactivity: Mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: CD34 antigen Calculated Molecular Weight: 41 kDa GenBank Accession Number: NM_133654 Gene Symbol: CD34 Gene ID (NCBI): 12490 RRID: AB_3669863 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q64314 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.