Proteintech, 31130-1-AP, Prealbumin/transthyretin Polyclonal antibody

Size: 20ul / 150ul The Prealbumin/transthyretin (31130-1-AP) by Proteintech is a Polyclonal antibody targeting Prealbumin/transthyretin in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 31130-1-AP targets Prealbumin/transthyretin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human heart tissue, human plasma tissue, mouse brain tissue, mouse liver tissue, rat plasma tissue Positive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Transthyretin (TTR) is a plasma transport protein for thyroxine and retinol, through the association with retinol-binding protein. It is a homotetrameric protein synthesized mainly in liver, choroid plexus, retinal pigment epithelium, and pancreas.Within the CNS, TTR is the only known protein synthesized solely by the choroid plexus. Mutant and wildtype TTR give rise to various forms of amyloid deposition (amyloidosis). Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR),hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) and carpal tunnel syndrome type 1 (CTS1). In addition, positive immunostaining for TTR has been reported as a sensitive diagnostic marker of choroid plexus tumors. However Western blot analysis using specific rabbit anti-mouse TTR reveals that TTR is absent from the cortex, hippocampus, and cerebellum and present in the choroid plexus. (PMID: 22103483, 16698124) Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Eg0931 Product name: Recombinant Human Prealbumin/Transthyretin protein (His Tag) Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 21-147 aa of NM_000371.4 Sequence: GPTGTGESKCPLMVKVLDAVRGSPAINVAVHVFRKAADDTWEPFASGKTSESGELHGLTTEEEFVEGIYKVEIDTKSYWKALGISPFHEHAEVVFTANDSGPRRYTIAALLSPYSYSTTAVVTNPKE Predict reactive species Full Name: transthyretin Calculated Molecular Weight: 16 kDa Observed Molecular Weight: 15 kDa GenBank Accession Number: NM_000371.4 Gene Symbol: TTR Gene ID (NCBI): 7276 RRID: AB_3669866 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P02766 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.