Proteintech, 31139-1-AP, Phospho-POLR2A (Ser7) Polyclonal antibody

Size: 20ul / 100ul The Phospho-POLR2A (Ser7) (31139-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-POLR2A (Ser7) in WB, ELISA applications with reactivity to Human samples 31139-1-AP targets Phospho-POLR2A (Ser7) in WB, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: HeLa cells, λ phosphatase treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information DNA-directed RNA polymerase II subunit RPB1(POLR2A), is part of the core element of the basic RNA polymerase II transcription mechanism. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for the assembly of factors that regulate transcription initiation, elongation, termination, and mRNA processing. Specification Tested Reactivity: Human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa Calculated Molecular Weight: 217 kDa Observed Molecular Weight: 260 kDa GenBank Accession Number: NM_000937 Gene Symbol: POLR2A Gene ID (NCBI): 5430 RRID: AB_3669869 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P24928 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.