Proteintech, 31177-1-AP, RBM20 Polyclonal antibody

Size: 20ul / 150ul The RBM20 (31177-1-AP) by Proteintech is a Polyclonal antibody targeting RBM20 in IHC, ELISA applications with reactivity to human, mouse, rat samples 31177-1-AP targets RBM20 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive IHC detected in: rat heart tissue, mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information RBM20 (RNA binding motif protein 20) is a striated muscle-specific splicing factor that regulates alternative splicing of many cardiac genes encoding sarcomere and calcium-regulatory proteins (PMID: 36417486). RBM20 has been identified as a crucial RNA-binding protein that controls the splicing of TTN (PMID: 30547036). RBM20 is expressed in all striated muscles but is highest in cardiac tissue (PMID: 34575212). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag34322 Product name: Recombinant human RBM20 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 667-840 aa of NM_001134363 Sequence: ADWGNGRDSWEHSPYARREEERDPAPWRDNGDDKRDRMDPWAHDRKHHPRQLDKAELDERPEGGRPHREKYPRSGSPNLPHSVSSYKSREDGYYRKEPKAKSDKYLKQQQDAPGRSRRKDEARLRESRHPHPDDSGKEDGLGPKVTRAPEGAKAKQNEKNKTKRTDRDQEGADD Predict reactive species Full Name: RNA binding motif protein 20 Calculated Molecular Weight: 134 kDa GenBank Accession Number: NM_001134363 Gene Symbol: RBM20 Gene ID (NCBI): 282996 RRID: AB_3669884 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q5T481 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.