Proteintech, 31234-1-AP, RC3H1 Polyclonal antibody

Size: 20ul / 150ul The RC3H1 (31234-1-AP) by Proteintech is a Polyclonal antibody targeting RC3H1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 31234-1-AP targets RC3H1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: 4T1 cells, HEK-293T cells, HeLa cells Positive IHC detected in: human ovary cancer tissue, mouse pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Roquin-1 (Rc3h1) is an E3 ubiquitin ligase originally discovered in a mutational screen for genetic factors contributory to systemic lupus erythematosus-like symptoms in mice. A single base pair mutation in the Rc3h1 gene resulted in the manifestation of autoantibody production and sustained immunological inflammation characterized by excessive T follicular helper cell activation and formation of germinal centers. (PMID: 26673963) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag35089 Product name: Recombinant human RC3H1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1000-1133 aa of NM_001300850 Sequence: NTLAGQSQPPPPPPPKWPGMISSEQLSLELHQVEREIGKRTRELSMENQCSLDMKSKLNTSKQAENGQPEPQNKVPAEDLTLTFSDVPNGSALTQENISLLSNKTSSLNLSEDPEGGGDNNDSQRSGVTPSSAP Predict reactive species Full Name: ring finger and CCCH-type zinc finger domains 1 Calculated Molecular Weight: 126 kDa Observed Molecular Weight: 130-150 kDa GenBank Accession Number: NM_001300850 Gene Symbol: RC3H1 Gene ID (NCBI): 149041 RRID: AB_3669912 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q5TC82 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.