Proteintech, 31251-1-AP, C-Reactive Protein/CRP Polyclonal antibody

Size: 20ul / 150ul The C-Reactive Protein/CRP (31251-1-AP) by Proteintech is a Polyclonal antibody targeting C-Reactive Protein/CRP in WB, IHC, ELISA applications with reactivity to rat samples 31251-1-AP targets C-Reactive Protein/CRP in WB, IHC, ELISA applications and shows reactivity with rat samples. Tested Applications Positive WB detected in: rat liver tissue Positive IHC detected in: rat liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein belonging to the pentraxin family. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis, and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation. This antibody is specific for rat Crp. Specification Tested Reactivity: rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Eg0942 Product name: Recombinant rat C-reactive protein protein Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 20-230 aa of NM_017096.3 Sequence: HEDMSKQAFVFPGVSATAYVSLEAESKKPLEAFTVCLYAHADVSRSFSIFSYATKTSFNEILLFWTRGQGFSIAVGGPEILFSASEIPEVPTHICATWESATGIVELWLDGKPRVRKSLQKGYIVGTNASIILGQEQDSYGGGFDANQSLVGDIGDVNMWDFVLSPEQINAVYVGRVFSPNVLNWRALKYETHGDVFIKPQLWPLTDCCES Predict reactive species Full Name: C-reactive protein, pentraxin-related Calculated Molecular Weight: 25 kDa Observed Molecular Weight: 25-28 kDa GenBank Accession Number: NM_017096.3 Gene Symbol: Crp Gene ID (NCBI): 25419 RRID: AB_3669919 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P48199 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.