Proteintech, 31291-1-AP, Tyrosinase Polyclonal antibody

Size: 20ul / 150ul The Tyrosinase (31291-1-AP) by Proteintech is a Polyclonal antibody targeting Tyrosinase in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 31291-1-AP targets Tyrosinase in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A375 cells, mouse liver tissue, mouse skin tissue, rat skin tissue Positive IHC detected in: human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information Tyrosinase is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. It's a melanocyte-specific enzyme critical for the synthesis of melanin, a process normally restricted to a post-Golgi compartment termed the melanosome. Loss-of-function mutations in tyrosinase are the cause of oculocutaneous albinism. The 60-kDa tyrosinase core polypeptide is modified in the endoplasmic reticulum (ER) by cotranslational addition of multiple N-linked glycans, producing the 70-kDa species. Complex sugar modifications in the Golgi apparatus further increases tyrosinase's molecular mass to 80 kDa, the size of the mature wild-type (WT) isoform. (PMID:10823941) Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag34940 Product name: Recombinant human Tyrosinase protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 19-200 aa of BC027179 Sequence: HFPRACVSSKNLMEKECCPPWSGDRSPCGQLSGRGSCQNILLSNAPLGPQFPFTGVDDRESWPSVFYNRTCQCSGNFMGFNCGNCKFGFWGPNCTERRLLVRRNIFDLSAPEKDKFFAYLTLAKHTISSDYVIPIGTYGQMKNGSTPMFNDINIYDLFVWMHYYVSMDALLGGSEIWRDIDF Predict reactive species Full Name: tyrosinase (oculocutaneous albinism IA) Calculated Molecular Weight: 529 aa, 60 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC027179 Gene Symbol: TYR Gene ID (NCBI): 7299 RRID: AB_3669932 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: P14679 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.