Proteintech, 31313-1-AP, Pan-cadherin Polyclonal antibody

Size: 20ul / 150ul The Pan-cadherin (31313-1-AP) by Proteintech is a Polyclonal antibody targeting Pan-cadherin in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 31313-1-AP targets Pan-cadherin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, C6 cells, HUVEC cells, HeLa cells, MCF-7 cells, mouse brain tissue, mouse heart tissue, human placenta tissue Positive IHC detected in: mouse colon tissue, mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in tissue morphogenesis and the maintenance of normal tissue architecture (PMID: 23885883). The classic cadherin family members include E-, N-, P-, R-, and B-cadherins. The classic cadherins have ectodomains composed of five extracellular cadherin (EC) repeats, a single transmembrane region, and a cytoplasmic domain. Structural characterization indicated that the known cadherins are glycoproteins ranging in their apparent molecular weights between 120 and 140 kDa (PMID: 2077036). This polyclonal antibody is raised against a synthetic peptide corresponding to a conserved region of human E-cadherin and can detect E-, N-, P-, VE-, and R-cadherins. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: cadherin 1, type 1, E-cadherin (epithelial) Calculated Molecular Weight: 97 kDa Observed Molecular Weight: 120-130 kDa GenBank Accession Number: NM_004360 Gene Symbol: E-cadherin Gene ID (NCBI): 999 RRID: AB_3669943 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: P12830 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.