Proteintech, 31684-1-AP, ARFGEF1 Polyclonal antibody

Size: 20ul / 150ul The ARFGEF1 (31684-1-AP) by Proteintech is a Polyclonal antibody targeting ARFGEF1 in WB, IHC, ELISA applications with reactivity to human, mouse samples 31684-1-AP targets ARFGEF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: BxPC-3 cells, HEK-293T cells, HeLa cells, NIH/3T3 cells, mouse lung tissue Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information ARFGEF1 (ADP ribosylation factor guanine nucleotide exchange factor 1), also known as BIG1 (Brefeldin A-inhibited guanine nucleotide exchange protein 1), is one of the ADP ribosylation factor (ARF) guanine nucleotide exchange factor (GEF) with a conserved catalytic sec7 domain.ARFGEF1 is connected with trafficking regulation through the trans-Golgi network and endosomes (PMID: 35090882). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag35629 Product name: Recombinant human ARFGEF1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 212-420 aa of NM_001413184 Sequence: QALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSLQDDTEPENGSDISSAENEQTEADQATAAETLSKNEVLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQ Predict reactive species Full Name: ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) Calculated Molecular Weight: 208kDa Observed Molecular Weight: 210 kDa GenBank Accession Number: NM_001413184 Gene Symbol: ARFGEF1 Gene ID (NCBI): 10565 RRID: AB_3670078 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q9Y6D6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.