Proteintech, 31808-1-AP, SPATA18 Polyclonal antibody

Size: 20ul / 150ul The SPATA18 (31808-1-AP) by Proteintech is a Polyclonal antibody targeting SPATA18 in WB, IHC, ELISA applications with reactivity to human, mouse samples 31808-1-AP targets SPATA18 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse testis tissue Positive IHC detected in: rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information The modulation of mitophagy after DNA damage is independent of the type of DNA damage stimuli used and the protein spermatogenesis-associated protein 18 (SPATA18) is an important player in this process (PMID: 32453416; 21264221). SPATA18, also known as MIEAP, is a p53 transcriptional target and was previously reported to contribute to mitochondrial quality control by inducing intramitochondrial lysosome-like organelles after mitochondrial damage (PMID: 21264221). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag36227 Product name: Recombinant human SPATA18 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 102-332 aa of BC025396 Sequence: LQDTFDRERHKDPSPRDRDMQQLDSNLNSTRSQCNQVQDDLVETEKNLEESKNRSAISLLAAEEEINQLKKQLKSLQAQEDARHRNTDQRSSENRRSEPWSLEERKREQWNSLKQNADQQDTEAMSDYKKQLRNLKEEIAVLSAEKSALQGRSSRSRSPSPAPRSRSCSRSRSASPSTAVKVRRPSPNRSKLSNVARKAALLSRFSDSYSQARLDAQCLLRRCIDKAETVQ Predict reactive species Full Name: spermatogenesis associated 18 homolog (rat) Calculated Molecular Weight: 538 aa, 61 kDa Observed Molecular Weight: 61 kDa GenBank Accession Number: BC025396 Gene Symbol: SPATA18 Gene ID (NCBI): 132671 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q8TC71 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.