Proteintech, 31833-1-AP, IK Polyclonal antibody

Size: 20ul / 150ul The IK (31833-1-AP) by Proteintech is a Polyclonal antibody targeting IK in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 31833-1-AP targets IK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293T cells, HeLa cells, HepG2 cells, SH-SY5Y cells Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1500-1:6000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information IK, also known as the nuclear protein RED, is a down-regulator of human leukocyte antigen (HLA) II and interacts with the human SAC protein MAD1, which is required for the localization of the kinetochore of MAD1, for the progression of mitosis and for the activation of the SAC. IK is therefore an important protein for the correct progression of mitosis through the regulation of mitotic kinases and phosphatases. In addition, IK may be involved in pre-mRNA splicing, which may be a useful biomarker for new nuclear structures in cells. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag37095 Product name: Recombinant human IK protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC013005 Sequence: MPERDSEPFSNPLAPDGHDVDDPHSFHQSKLTNEDFRKLLMTPRAAPTSAPPSKSRHHEMPREYNEDEDPAARRRKKKSYYAKLRQQEIERERELAEKYRDRAKERRDGVNKDYEETELISTTANYRAVGPTAEADKSAAEKRRQLIQESKFLGGDMEHTHLVKGLDFALLQKVRAEIASKEKEEEELMEKPQKETKKDEDPENKIEFKTRLGRNV Predict reactive species Full Name: IK cytokine, down-regulator of HLA II Calculated Molecular Weight: 557 aa, 66 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC013005 Gene Symbol: IK Gene ID (NCBI): 3550 RRID: AB_3670123 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q13123 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.