Proteintech, 31938-1-AP, ARHGAP23 Polyclonal antibody

Size: 20ul / 150ul The ARHGAP23 (31938-1-AP) by Proteintech is a Polyclonal antibody targeting ARHGAP23 in WB, IHC, ELISA applications with reactivity to human, mouse samples 31938-1-AP targets ARHGAP23 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A549 cells, Calu-1 cells, HeLa cells Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information ARHGAP23 (Rho GTPase Activating Protein 23) belongs to the family of Rho GTPase activating proteins. The protein functions as a GTPase-activating factor by converting Rho class GTPases into their inactive GDP-bound state. ARHGAP23 is involved in the Rho GTPase signaling pathway, affecting processes such as cytoskeletal reorganization, cell migration, and cell proliferation. In non-small cell lung cancer, the expression of ARHGAP23 is associated with tumor immune infiltration and prognosis, and its role in tumor development has attracted attention. Mutations in this gene are related to a variety of diseases, including certain types of cancer and neurological disorders. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag36340 Product name: Recombinant human ARHGAP23 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 500-700 aa of NM_001199417 Sequence: KVQLTPARQMNLGFGDESPEPEASGRGERLGRKVAPLATTEDSLASIPFIDEPTSPSIDLQAKHVPASAVVSSAMNSAPVLGTSPSSPTFTFTLGRHYSQDCSSIKAGRRSSYLLAITTERSKSCDDGLNTFRDEGRVLRRLPNRIPSLRMLRSFFTDGSLDSWGTSEDADAPSKRHSTSDLSDATFSDIRREGWLYYKQI Predict reactive species Full Name: Rho GTPase activating protein 23 Calculated Molecular Weight: 162kDa Observed Molecular Weight: 140-150 kDa GenBank Accession Number: NM_001199417 Gene Symbol: ARHGAP23 Gene ID (NCBI): 57636 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q9P227 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.