Proteintech, 32112-1-AP, PWWP2B Polyclonal antibody

Size: 20ul / 150ul The PWWP2B (32112-1-AP) by Proteintech is a Polyclonal antibody targeting PWWP2B in WB, ELISA applications with reactivity to human samples 32112-1-AP targets PWWP2B in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, BxPC-3 cells, HaCaT cells, HeLa cells, LNCaP cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Background Information PWWP2B (PWWP structural domain protein 2B) is a PWWP structural domain-containing protein involved in a variety of cellular functions and biological processes. PWWP2B is highly expressed in brown and beige adipose tissue and its expression is induced by cold stimulation. It has been shown that PWWP2B negatively regulates adipose tissue thermogenesis by recruiting and stabilizing the histone deacetylase HDAC1/2, which binds to the promoter regions of thermogenic genes and reduces histone acetylation levels. In adipocytes, PWWP2B acts as part of the nucleosome remodeling and deacetylation complex (NuRD) to inhibit the expression of thermogenic genes and avoid overactivation of thermogenic functions. In addition, PWWP2B plays an important role in DNA damage repair, as its PWWP structural domain moves to DNA damage sites, interacts with UHRF1 and recruits MRE11 to promote DNA end resection and homologous recombination repair. In pathology, downregulation of PWWP2B is associated with recurrent gastric cancer and promotes cell proliferation and migration. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag36839 Product name: Recombinant human PWWP2B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 199-413 aa of BC068574 Sequence: SLEPFRPQQAPQDDGSQDPEVLDRESRDRPSCAPSASIPKLKLTRPVPAGADLPPPKIRLKPHRLGDSEHEPVYRAELVGELNGYLRDSSPAPCADGPAGGLADLSSGSSGEDDDFKSCPQGPQGREGLAFLVSCPEGRADCASESACSSDSLDEARSSGSEGTPADTGDLSPGHGASAPSVSREARQTVPPLTVRLHTQSVSECITEDGRTVAV Predict reactive species Full Name: PWWP domain containing 2B Observed Molecular Weight: 90 kDa GenBank Accession Number: BC068574 Gene Symbol: PWWP2B Gene ID (NCBI): 170394 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q6NUJ5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.