Proteintech, 32113-1-AP, NSMCE1 Polyclonal antibody

Size: 20ul / 150ul The NSMCE1 (32113-1-AP) by Proteintech is a Polyclonal antibody targeting NSMCE1 in WB, ELISA applications with reactivity to human samples 32113-1-AP targets NSMCE1 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HL-60 cells, HepG2 cells, LNCaP cells, Raji cells, U-87 MG cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information Non-structural maintenance of chromosomes element 1 homolog (NSMCE1, also known as NSE1) located in the nucleoplasm, which involves the maintenance of genome integrity, DNA damage response, and DNA repair (PMID: 18086888; 20864041; 29225034). NSMCE3/MAGEG1 and NSMCE1 ubiquitin ligase are components of the SMC5-SMC6 complex and may positively regulate homologous recombination-mediated DNA repair (PMID: 18086888). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag36841 Product name: Recombinant human NSMCE1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-260 aa of BC018938 Sequence: RMGVMTDVHRRFLQLLMTHGVLEEWDVKRLQTHCYKVHDRSATVDKLEDFINNINSVLESLYIEIKRGVTEDDGRPIYALVNLATTSISKMATDFAENELDLFRKALELIIDSETGFASSTNILNLVDQLKGKKMRKKEAEQVLQKFVQNKWLIEKEGEFTLHGRAILEMEQYIRETYPDAVKICNICHSLLIQGQSCETCGIRMHLPCVAKYFQSNAEPRCPHCNDYWPHEIPKVFDPEKERESGVLKSNKKSLRSRQH Predict reactive species Full Name: non-SMC element 1 homolog (S. cerevisiae) Calculated Molecular Weight: 31 kDa Observed Molecular Weight: 31 kDa GenBank Accession Number: BC018938 Gene Symbol: NSMCE1 Gene ID (NCBI): 197370 RRID: AB_3670196 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q8WV22 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.