Proteintech, 32134-1-AP, EPCR/CD201 Polyclonal antibody

Size: 20ul / 150ul The EPCR/CD201 (32134-1-AP) by Proteintech is a Polyclonal antibody targeting EPCR/CD201 in WB, IHC, ELISA applications with reactivity to human, mouse samples 32134-1-AP targets EPCR/CD201 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: THP-1 cells, bEnd.3 cells Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information Protein C receptor (PROCR), also known as activated protein C receptor (APC receptor), EPCR, or CD201, is a transmembrane glycoprotein. EPCR promotes the activation of protein C, which has anticoagulant and cytoprotective effects. EPCR facilitates the activation of protein C by the thrombin-thrombomodulin complex but also facilitates APC-mediated cytoprotective effects on cells that involve the activation of protease-activated receptors (PAR) 1 and 3(PMID: 27207424). EPCR is approximately 46 kDa, truncated EPCR is 23 kDa, truncated EPCR with the transmembrane domain is 26 kDa, and soluble EPCR is 43 kDa (PMID: 11686350). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: protein C receptor, endothelial Calculated Molecular Weight: 27kDa Observed Molecular Weight: 27 kDa, 40-50 kDa GenBank Accession Number: NM_011171.2 Gene Symbol: PROCR Gene ID (NCBI): 19124 RRID: AB_3670204 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q64695 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.