Proteintech, 32150-1-AP, CD24 Polyclonal antibody

Size: 20ul / 150ul The CD24 (32150-1-AP) by Proteintech is a Polyclonal antibody targeting CD24 in WB, IHC, ELISA applications with reactivity to mouse samples 32150-1-AP targets CD24 in WB, IHC, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: mouse liver tissue, mouse spleen tissue Positive IHC detected in: mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009) Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Eg2104 Product name: Recombinant Mouse CD24 protein (rFc Tag)(HPLC verified) Source: mammalian cells -derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 27-53 aa of NM_009846.2 Sequence: NQTSVAPFPGNQNISASPNPSNATTRG Predict reactive species Full Name: CD24a antigen Calculated Molecular Weight: 8 kDa Observed Molecular Weight: 50-55 kDa GenBank Accession Number: NM_009846.2 Gene Symbol: CD24 Gene ID (NCBI): 12484 RRID: AB_3670209 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: P24807 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.