Proteintech, 32217-1-AP, GCC2 Polyclonal antibody

Size: 20ul / 150ul The GCC2 (32217-1-AP) by Proteintech is a Polyclonal antibody targeting GCC2 in WB, IHC, ELISA applications with reactivity to human samples 32217-1-AP targets GCC2 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293T cells, HeLa cells, Jurkat cells, Raji cells Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Background Information The GCC2 protein, fully named GRIP and Coiled-Coil Domain-Containing Protein 2, is a key adaptor protein in the intracellular Golgi-endosomal membrane transport system. It specifically anchors to the Golgi membrane via its GRIP domain and is primarily responsible for mediating retrograde vesicular transport from endosomes to the Golgi apparatus, participating in the recycling and sorting of various cargo proteins, such as the mannose-6-phosphate receptor. Recent studies have revealed that GCC2 regulates the subcellular localization of the GATOR complex, thereby acting as an important upstream regulator of the mTORC1 signaling pathway, which influences cell growth, metabolism, and autophagy. Dysfunction of GCC2 is closely associated with neurodevelopmental disorders and tumorigenesis, underscoring its significance in cell biology and disease research. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag17535 Product name: Recombinant human GCC2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-343 aa of BC146789 Sequence: MKQEVEDSVTKMGDAHKELEQSHINYVKEIENLKNELMAVRSKYSEDKANLQKQLEEAMNTQLELSEQLKFQNNSEDNVKKLQEEIEKIRPGFEEQILYLQKQLDATTDEKKETVTQLQNIIEANSQHYQKNINSLQEELLQLKAIHQEEVKELMCQIEASAKEHEAEINKLNELKENLVKQCEASEKNIQKKYECELENLRKATSNANQDNQICSILLQENTFVEQVVNEKVKHLEDTLKELESQHSILKDEVTYMNNLKLKLEMDAQHIKDEFFHEREDLEFKINELLLAKEEQGCVIEKLKSELAGLNKQFCYTVEQHNREVQSLKEQHQKEISELNETF Predict reactive species Full Name: GRIP and coiled-coil domain containing 2 Calculated Molecular Weight: 1583 aa, 185 kDa Observed Molecular Weight: 200 kDa GenBank Accession Number: BC146789 Gene Symbol: GCC2 Gene ID (NCBI): 9648 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q8IWJ2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.