Proteintech, 32277-1-AP, SAV1 Polyclonal antibody

Size: 20ul / 150ul The SAV1 (32277-1-AP) by Proteintech is a Polyclonal antibody targeting SAV1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 32277-1-AP targets SAV1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HT-29 cells, HUVEC cells, U2OS cells Positive IHC detected in: mouse kidney tissue, rat kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information The Salvador family WW domain‐containing 1 (SAV1, also known as WW45) is a human homologue of Salvador that contains two protein‐protein interaction modules known as WW domains and acts as a scaffolding protein (PMID: 28618450). SAV1 belongs to the core kinase components of Hippo signalling pathway for efficient MST1/2 activation. SAV1 represses the growth of colorectal cancer cells by inactivating Akt‐mTOR signalling in a YAP‐dependent manner (PMID: 29063833, 22185343). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag36278 Product name: Recombinant human SAV1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 208-383 aa of BC020537 Sequence: DWTMRGRKYYIDHNTNTTHWSHPLEREGLPPGWERVESSEFGTYYVDHTNKKAQYRHPCAPSVPRYDQPPPVTYQPQQTERNQSLLVPANPYHTAEIPDWLQVYARAPVKYDHILKWELFQLADLDTYQGMLKLLFMKELEQIVKMYEAYRQALLTELENRKQRQQWYAQQHGKNF Predict reactive species Full Name: salvador homolog 1 (Drosophila) Calculated Molecular Weight: 383 aa, 45 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: BC020537 Gene Symbol: SAV1 Gene ID (NCBI): 60485 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q9H4B6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.