Proteintech, 32519-1-AP, ZNF280B Polyclonal antibody

Size: 20ul / 150ul The ZNF280B (32519-1-AP) by Proteintech is a Polyclonal antibody targeting ZNF280B in WB, IP, ELISA applications with reactivity to human samples 32519-1-AP targets ZNF280B in WB, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: K-562 cells, U2OS cells Positive IP detected in: K-562 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information ZNF280B belongs to the family of zinc finger proteins, a transcription factor that is mainly involved in the regulation of gene expression. In prostate cancer cells, ZNF280B induces p53 nuclear export, leading to subsequent proteasomal degradation. Therefore, ZNF280B has pro-growth and pro-survival functions in prostate cancer cells. In addition to this, ZNF280B has a promotional role in gastric and lung cancer development, and its potential as a tumor therapeutic target can be explored in the future. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag38544 Product name: Recombinant human ZNF280B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 75-268 aa of NM_080764.3 Sequence: YDHLRKDTARKLQPKSHETVTSEAVTVLPASQLESRSTDSPIIIEPLSKPDYRNSSPQVVPNNSSELPSPLITFTDSLHHPVSTALSVGGINESPRVSKQLSTFEVNSINPKRAKLRDGIIEGNSSASFPSDTFHTMNTQQSTPSNNVHTSLSHVQNGAPFPAAFPKDNIHFKPINTNLDRANELAKTDILSLT Predict reactive species Full Name: zinc finger protein 280B Calculated Molecular Weight: 62kDa，543aa Observed Molecular Weight: 60 kDa GenBank Accession Number: NM_080764.3 Gene Symbol: ZNF280B Gene ID (NCBI): 140883 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q86YH2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.