Proteintech, 32873-1-AP, Mup1 Polyclonal antibody

Size: 20ul / 150ul The Mup1 (32873-1-AP) by Proteintech is a Polyclonal antibody targeting Mup1 in WB, ELISA applications with reactivity to mouse samples 32873-1-AP targets Mup1 in WB, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: mouse liver tissue, mouse serum Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information MUP1 is a low-molecular-weight secreted protein primarily produced by the liver and belongs to the lipocalin family. Lipocalin family members carry small hydrophobic ligands such as pheromones. However, the physiological functions of MUP1 remain poorly understood. Microarray and real-time PCR analyses revealed that MUP1 mRNA abundance in the livers of db/db obese mice was reduced approximately 30-fold compared to their lean littermates. This change was partially reversed by treatment with the insulin-sensitizing drug rosiglitazone. In both dietary and genetic obese mice, circulating MUP1 concentrations were significantly lower than in lean controls. Chronic elevation of circulating MUP1 in db/db mice using an osmotic pump-based protein delivery system increased energy expenditure and locomotor activity, raised core body temperature, and reduced glucose intolerance and insulin resistance. At the molecular level, MUP1-mediated improvements in metabolic profiles were accompanied by increased expression of genes involved in mitochondrial biogenesis, enhanced mitochondrial oxidative capacity, reduced triglyceride accumulation, and improved insulin-evoked Akt signaling in skeletal muscle (but not in the liver). These findings suggest that MUP1 deficiency may contribute to metabolic dysregulation in obese/diabetic mice and that the beneficial metabolic effects of MUP1 are partly attributed to its ability to enhance skeletal muscle mitochondrial function (PMID: 36455814). Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Eg3024 Product name: Recombinant Mouse Mup1 protein (rFc Tag) (HPLC verified) Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 19-180 aa of NM_001199995.1 Sequence: EEASSTGRNFNVEKINGEWHTIILASDKREKIEDNGNFRLFLEQIHVLENSLVLKFHTVRDEECSELSMVADKTEKAGEYSVTYDGFNTFTIPKTDYDNFLMAHLINEKDGETFQLMGLYGREPDLSSDIKERFAQLCEKHGILRENIIDLSNANRCLQARE Predict reactive species Full Name: major urinary protein 1 Calculated Molecular Weight: 21 kDa Observed Molecular Weight: 20 kDa GenBank Accession Number: NM_001199995.1 Gene Symbol: Mup1 Gene ID (NCBI): 17840 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: P11588 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.