Proteintech, 33113-1-AP, DENND2D Polyclonal antibody

Size: 20ul / 150ul The DENND2D (33113-1-AP) by Proteintech is a Polyclonal antibody targeting DENND2D in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 33113-1-AP targets DENND2D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, MCF-7 cells, MDA-MB-231 cells, MOLT-4 cells, SK-BR-3 cells Positive IHC detected in: human ovary tumor tissue, human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information DENND2D (DENN domain-containing protein 2D) is a protein containing a DENN domain and belongs to the family of membrane transport proteins, participating in intracellular membrane transport and signal transduction processes. The DENND2D protein comprises several conserved domains, such as the uDENN, DENN, and dDENN domains, which are widely present in various signaling proteins. In cancer research, the expression and methylation status of DENND2D hold significant prognostic value. DENND2D may serve as a novel tumor biomarker for predicting cancer progression and recurrence. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag36381 Product name: Recombinant human DENND2D protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 305-471 aa of BC004557 Sequence: FMVGVQMRFQQEVMDSPMEEVLLVNLCEGTFLMSVGDEKDILPPKLQDDILDSLGQGINELKTAEQINEHVSGPFVQFFVKIVGHYASYIKREANGQGHFQERSFCKALTSKTNRRFVKKFVKTQLFSLFIQEAEKSKNPPAGYFQQKILEYEEQKKQKKPREKTVK Predict reactive species Full Name: DENN/MADD domain containing 2D Observed Molecular Weight: 48-50 kDa GenBank Accession Number: BC004557 Gene Symbol: DENND2D Gene ID (NCBI): 79961 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q9H6A0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.