Proteintech, 33133-1-AP, BXDC5 Polyclonal antibody

Size: 20ul / 150ul The BXDC5 (33133-1-AP) by Proteintech is a Polyclonal antibody targeting BXDC5 in WB, IF/ICC, ELISA applications with reactivity to human samples 33133-1-AP targets BXDC5 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, U-251 cells, RKO cells, RT-4 cells Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information BXDC5 (Brix domain-containing protein 5), also known as ribosome biogenesis protein RPF1, is a protein containing a Brix domain and is localized in the nucleolus. The Brix domain is a region that shares homology with the yeast protein Pitx1 (ribosome biogenesis protein BRX1). BXDC5 is a key factor in ribosome biogenesis, participating in the maturation of the 60S ribosomal subunit. This protein exerts its function by binding to specific rRNA structures and acts as a downstream effector molecule of the Ran signaling pathway, involved in ribosome biogenesis. In colorectal cancer (CRC), BXDC5 influences the occurrence and development of tumors by regulating ribosome biogenesis. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag37505 Product name: Recombinant human BXDC5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-349 aa of BC016051 Sequence: MAKAGDKSGSSGKKSLKRKAAAEELQEAAGAGDGATENGVQPPKAAAFPPGFSISEIKNKQRRHLMFTRWKQQQRKEKLAAKKKLKKEREALGDKAPPKPVPKTIDNQRVYDETTVDPNDEEVAYDEATDEFASYFNKQTSPKILITTSDRPHGRTVRLCEQLSTVIPNSHVYYRRGLALKKIIPQCIARDFTDLIVINEDRKTPNGLILSHLPNGPTAHFKMSSVRLRKEIKRRGKDPTEHIPEIILNNFTTRLGHSIGRMFASLFPHNPQFIGRQVATFHNQRDYIFFRFHRYIFRSEKKVGIQELGPRFTLKLRSLQKGTFDSKYGEYEWVHKPREMDTSRRKFHL Predict reactive species Full Name: brix domain containing 5 Calculated Molecular Weight: 349 aa, 40 kDa Observed Molecular Weight: 48 kDa GenBank Accession Number: BC016051 Gene Symbol: BXDC5 Gene ID (NCBI): 80135 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q9H9Y2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.