Proteintech, 33223-1-AP, PTPRJ Polyclonal antibody

Size: 20ul / 150ul The PTPRJ (33223-1-AP) by Proteintech is a Polyclonal antibody targeting PTPRJ in WB, ELISA applications with reactivity to mouse samples 33223-1-AP targets PTPRJ in WB, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, mouse spleen tissue, NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Background Information PTPRJ, also named as CD148 and DEP1, belongs to the protein-tyrosine phosphatase family and Receptor class 3 subfamily. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPRJ may contribute to the mechanism of contact inhibition of cell growth. PTPRJ is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 (PLCG1) and Linker for Activation of T Cells (LAT). It was also found to dephosphorylate PDGF beta receptor, and may be involved in UV-induced signal transduction. Its observed molecular weight is reported as double bands, 220 kDa and 240 kDa (PMID: 33605542). Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Eg2648 Product name: Recombinant mouse Ptprj protein Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 29-874 aa of NM_008982.5 Sequence: RQGQVLCAGAAPNPIFDIEAVVSPTSVLLTWKHNDSGASECRIENKMESNLTFPVKNQTSCNITGLSPGTSYTFSIISVTTNETLNKTITTEPWPVSDLHVTSVGVTQARLTWSNANGTASYRMLIEELTTHSSVNISGLKPGTNNSFAFPESNETQADFAVAEEVPDANGTKRIPVTNLSQLHKNSLVSVDPPSGQDPSLTEILLTDLKPDTQYNATIYSQAANGTEGQPRNKVFKTNSTQVSDVRAMNISASSMTLTWKSNYDGSRTSIVYKIHVAGGTHSVNQTVNKTEAIILGLSSSTLYNITVHPFLGQTEGTPGFLQVYTSPDQVSDFRVTNVSTRAIGLAWRSNDSKSFEIFIKQDGGEKHRNASTGNQSYMVEDLKPGTSYHFEIIPRGPDGTEGLSSTVNGSTDPSAVTDIRVVNISTTEMQLEWQNTDDASGYTYHLVLESKSGSIIRTNSSQKWITVGSLTPGTLYNVTIFPEVDQIQGISNSITQYTRPSSVSHIEVNTTTTTAAIRWKNEDAASASYAYSVLILKTGDGSNVTSNFTKDPSILIPELIPGVSYTVKILTQVGDGTTSLVPGWNLFCTEPEPVTSFHCEVVPKEPALVLKWACPFGMYTGFELGVRSDSWDNMTRLENCTSDDDTECRTEVAYLNFSTSYNISIATLSCGKMALPAQNICTTGITDPPTPDGSPNITSVSHNSVKVKFSGFEASHGPIKAYAVILTTGEAAQPSADVLKYTYEDFKRGASDTYVTYLIRIEEKGQSQGLSEVLNYEIDVGNQSTTLGYYNGRLEPLGSYRACVAGFTNITYNLQNDGLINGDESYVSFSPYSEAVFLPQDPGVI Predict reactive species Full Name: protein tyrosine phosphatase, receptor type, J Calculated Molecular Weight: 137 kDa Observed Molecular Weight: 220 kDa, 240 kDa GenBank Accession Number: NM_008982.5 Gene Symbol: Ptprj Gene ID (NCBI): 19271 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q64455 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.