Proteintech, 33263-1-AP, SLC25A31/ANT4 Polyclonal antibody

Size: 20ul / 150ul The SLC25A31/ANT4 (33263-1-AP) by Proteintech is a Polyclonal antibody targeting SLC25A31/ANT4 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 33263-1-AP targets SLC25A31/ANT4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human testis tissue, mouse testis tissue, rat testis tissue Positive IHC detected in: mouse kidney tissue, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U-251 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information SLC25A31, also known as ANT4, is a member of the mitochondrial carrier family. The proteins of this family are mainly involved in the transport of substances inside and outside the mitochondria. SLC25A31 is an ADP/ATP antiporter protein, which catalyzes the exchange of ATP generated in mitochondria by ATP synthase against ADP produced in cytosol by most energy-consuming reactions(PMID: 15670820). SLC25A31 is mainly expressed in the testis and male germ cells (PMID: 25031318, 23506884). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11008 Product name: Recombinant human SLC25A31 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-315 aa of BC022032 Sequence: MHREPAKKKAEKRLFDASSFGKDLLAGGVAAAVSKTAVAPIERVKLLLQVQASSKQISPEARYKGMVDCLVRIPREQGFFSFWRGNLANVIRYFPTQALNFAFKDKYKQLFMSGVNKEKQFWRWFLANLASGGAAGATSLCVVYPLDFARTRLGVDIGKGPEERQFKGLGDCIMKIAKSDGIAGLYQGFGVSVQGIIVYRASYFGAYDTVKGLLPKPKKTPFLVSFFIAQVVTTCSGILSYPFDTVRRRMMMQSGEAKRQYKGTLDCFVKIYQHEGISSFFRGAFSNVLRGTGGALVLVLYDKIKEFFHIDIGGR Predict reactive species Full Name: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 31 Calculated Molecular Weight: 315 aa, 35 kDa Observed Molecular Weight: 35 kDa GenBank Accession Number: BC022032 Gene Symbol: SLC25A31 Gene ID (NCBI): 83447 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q9H0C2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.