Proteintech, 33332-1-AP, Mrgprx1 Polyclonal antibody

Size: 20ul / 150ul The Mrgprx1 (33332-1-AP) by Proteintech is a Polyclonal antibody targeting Mrgprx1 in WB, ELISA applications with reactivity to mouse, rat samples 33332-1-AP targets Mrgprx1 in applications and shows reactivity with mouse, rat samples. Tested Applications Positive WB detected in: mouse liver tissue, rat heart tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information MRGPRX1 is a MAS-related G protein-coupled receptor (GPCR) primarily expressed in dorsal root ganglion (DRG) neurons and is a key receptor for itch perception. It can be activated by various endogenous peptides and exogenous ligands, triggering itch and pain signals through Gi and Gq signaling pathways. The activation of MRGPRX1 not only induces the sensation of itch in peripheral neurons but also inhibits neurotransmitter release in the spinal cord via the Gi1 pathway, alleviating chronic pain. Additionally, the structure and activation mechanism of MRGPRX1 have been elucidated, with its unique ligand-binding pocket and activation process providing a theoretical basis for the development of novel analgesic and antipruritic drugs. Specification Tested Reactivity: mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: MAS-related GPR, member X1 Calculated Molecular Weight: 322aa, 37 kDa Observed Molecular Weight: 35 kDa GenBank Accession Number: NM_207540 Gene Symbol: Mrgprx1 Gene ID (NCBI): 404242 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q8CIP3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.