Proteintech, 33578-1-AP, FBXW8 Polyclonal antibody

Size: 20ul / 150ul The FBXW8 (33578-1-AP) by Proteintech is a Polyclonal antibody targeting FBXW8 in WB, ELISA applications with reactivity to human samples 33578-1-AP targets FBXW8 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MCF-7 cells, MDA-MB-231 cell, U-87 MG cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information Fbxw8 (also known as Fbw6 or Fbx29) is an F-box protein that is assembled with Cul7 in an SCF-like E3 complex. FBXW8 directs the proteasomal degradation of β-TrCP1 in MAPK pathway-dependent manner, and the attenuation of β-TrCP1 by FBXW8 is important for Cdc25A-mediated cell cycle transition from G1 phase to S phase as well as DNA damage-free progression of S phase (PMID: 33742524). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag36986 Product name: Recombinant human FBXW8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-303 aa of BC037296 Sequence: MDDYSLDEFRRRWQEELAQAQAPKKRRRPEAAERRARRPEVGSGRSEQASGDPALAQGLLEGAGRPPAARATRAEGQDVASRSRSPLAREGAGGGEQLVDQLIRDLNEMNDVPFFDIQLPYELAINIFQYLDRKELGRCAQVSKTWKVIAEDEVLWYRLCQQEGHLPDSSISDYSCWKLIFQECRAKEHMLQTNWKNRKGAVSELEHVPDTVLCDVHSHDGVVIAGYTSGDVRVWDTRTWDYVAPFLESEDEEDEPGMQPNVSFVRINSSLAVAAYEDGFLNIWDLRTGKYPVHRFEHDARIQ Predict reactive species Full Name: F-box and WD repeat domain containing 8 Calculated Molecular Weight: 67 kDa Observed Molecular Weight: 67 kDa, 60 kDa GenBank Accession Number: BC037296 Gene Symbol: FBXW8 Gene ID (NCBI): 26259 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q8N3Y1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.