Proteintech, 33828-1-AP, CXCR4/CD184 Polyclonal antibody

Size: 20ul / 150ul The CXCR4/CD184 (33828-1-AP) by Proteintech is a Polyclonal antibody targeting CXCR4/CD184 in WB, ELISA applications with reactivity to human, mouse, rat samples 33828-1-AP targets CXCR4/CD184 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, mouse spleen tissue, rat brain tissue, rat spleen tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some research, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag40645 Product name: Recombinant human CXCR4/CD184 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 303-352 aa of BC020968 Sequence: AFLGAKFKTSAQHALTSVSRGSSLKILSKGKRGGHSSVSTESESSSFHSS Predict reactive species Full Name: chemokine (C-X-C motif) receptor 4 Calculated Molecular Weight: 352 aa, 40 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC020968 Gene Symbol: CXCR4 Gene ID (NCBI): 7852 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: P61073 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.