Proteintech, 51045-1-AP, Snf1lk Polyclonal antibody

Size: 20ul / 150ul The Snf1lk (51045-1-AP) by Proteintech is a Polyclonal antibody targeting Snf1lk in IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 51045-1-AP targets Snf1lk in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IHC detected in: mouse brain tissue, human liver tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information SIK1(Salt-inducible kinase 1) is also named SIK, SNF1LK, and belongs to the CAMK Ser/Thr protein kinase family. It associates constitutively with the NK regulatory complex and is responsible for increases in its catalytic activity following small elevations in intracellular sodium concentrations(PMID:17939993). SIK1 is required for the phosphorylation of class II HDACs and the expression of MEF2 target genes in myocytes(PMID:17468767). Defects in SIK1 may be associated with some cancers, such as breast cancer. Loss of SIK1 correlates with poor patient outcomes in breast cancers(PMID:19622832). SIK1 also can be detected 95-100 kDa (PMID: 26567857, PMID: 22109884). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3913 Product name: Recombinant mouse Snf1lk protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 423-769 aa of NM_010831 Sequence: LDTNCSGVFRHRSISPSSLLDTAISEEARQGPSLEEEQEVQEPLPGSTGRRHTLAEVSTHFSPLNPPCIIVSSSATASPSEGTSSDSCLPFSASEGPAGLGSGLATPGLLGTSSPVRLASPFLGSQSATPVLQTQAGLGTAVLPPVSFQEGRRASDTSLTQGLKAFRQQLRKNARTKGFLGLNKIKGLARQVCQSSVRTPRGGMSTFHTPAPSSGLQGCTTSNREGRSLLEEVLHQQRLLQLQHHSSTAAASSGCQQGPQLSPVPYVLAPCDSLLVSGIPLLPTPLLQAGMSPVASAAHLLDTHLHISAGPVALPTGPLPQCLTRLSPGCDPAGLPQGDCEMEDLTS Predict reactive species Full Name: salt inducible kinase 1 Calculated Molecular Weight: 85 kDa Observed Molecular Weight: 85-100 kDa GenBank Accession Number: NM_010831 Gene Symbol: Sik1 Gene ID (NCBI): 17691 RRID: AB_2187322 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q60670 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.