Proteintech, 51073-1-AP, STAT6 Polyclonal antibody

Size: 20ul / 150ul The STAT6 (51073-1-AP) by Proteintech is a Polyclonal antibody targeting STAT6 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 51073-1-AP targets STAT6 in WB, IHC, IF, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, NIH/3T3 cells, THP-1 cells, Jurkat cells, PC-12 cells Positive IHC detected in: human spleen tissue, human lung tissue, mouse lung tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling. The antibody could identify about 100 kDa band, similar to the reported in the literature(PMID: 10961983). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: signal transducer and activator of transcription 6, interleukin-4 induced Calculated Molecular Weight: 94 kDa Observed Molecular Weight: 100-110 kDa GenBank Accession Number: BC075852 Gene Symbol: STAT6 Gene ID (NCBI): 6778 RRID: AB_2197244 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P42226 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.