Proteintech, 55013-1-AP, c-MAF Polyclonal antibody

Size: 20ul / 150ul The c-MAF (55013-1-AP) by Proteintech is a Polyclonal antibody targeting c-MAF in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 55013-1-AP targets c-MAF in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, A375 cells, HeLa cells, HepG2 cells, K-562 cells Positive IP detected in: A431 cells Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information MAF, also named as c-Maf, belongs to the bZIP family and Maf subfamily. MAF acts as a transcriptional activator or repressor. It is involved in embryonic lens fiber cell development. MAF increases T cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, it transactivates strongly the glucagon gene promoter through the G1 element. MAF activates transcription of the CD13 proximal promoter in endothelial cells. It is involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. When overexpressed, MAF represses anti-oxidant reponse element (ARE)-mediated transcription. It is involved either as an oncogene or as a tumor suppressor, depending on the cell context. A chromosomal aberration involving MAF is found in some forms of multiple myeloma (MM). Defects in MAF are the cause of cataract pulverulent juvenile-onset MAF-related (CAPJOM). Defects in MAF are the cause of cataract congenital cerulean type 4 (CCA4). The antibody is specific to MAF. And it could recognise the 50 kDa band that also be detected in the paper (PMID: 25770584 ) . Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian) Calculated Molecular Weight: 42 kDa Observed Molecular Weight: 42-52 kDa GenBank Accession Number: NM_005360 Gene Symbol: MAF Gene ID (NCBI): 4094 RRID: AB_10863127 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75444 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.