Proteintech, 55041-1-AP, SOS1 Polyclonal antibody

Size: 20ul / 150ul The SOS1 (55041-1-AP) by Proteintech is a Polyclonal antibody targeting SOS1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 55041-1-AP targets SOS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: K-562 cells, mouse brain tissue, THP-1 cells, SKOV-3 cells Positive IP detected in: mouse brain tissue Positive IHC detected in: mouse colon tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information SOS1 promotes the exchange of Ras-bound GDP by GTP. EPS8, E3B1 and SOS1 form a tricomplex in vivo that exhibits RAC-specific guanine nucleotide exchange factor activity in vitro. Defects in SOS1 are the cause of gingival fibromatosis 1 (GGF1). Defects in SOS1 are the cause of Noonan syndrome type 4 (NS4). The antibody is specific to SOS1. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: son of sevenless homolog 1 (Drosophila) Calculated Molecular Weight: 152 kDa Observed Molecular Weight: 150-170 kDa GenBank Accession Number: NM_005633 Gene Symbol: SOS1 Gene ID (NCBI): 6654 RRID: AB_10804659 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q07889 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.