Proteintech, 55059-1-AP, SHANK1 Polyclonal antibody

Size: 20ul / 150ul The SHANK1 (55059-1-AP) by Proteintech is a Polyclonal antibody targeting SHANK1 in WB, IF-P, IP, ELISA applications with reactivity to human, mouse, rat samples 55059-1-AP targets SHANK1 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293T cells, Neuro-2a cells Positive IP detected in: HEK-293T cells Positive IF-P detected in: mouse eye tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information SHANK1 belongs to the SHANK family. It seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. SHANK1 may play a role in the structural and functional organization of the dendritic spine and synaptic junction. The antibody is specific to SHANK1. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: SH3 and multiple ankyrin repeat domains 1 Calculated Molecular Weight: 225 kDa Observed Molecular Weight: 159-225 kDa GenBank Accession Number: NM_016148 Gene Symbol: SHANK1 Gene ID (NCBI): 50944 RRID: AB_10863130 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y566 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.