Proteintech, 55117-1-AP, EGR1 Polyclonal antibody

Size: 20ul / 150ul The EGR1 (55117-1-AP) by Proteintech is a Polyclonal antibody targeting EGR1 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications with reactivity to human, mouse samples 55117-1-AP targets EGR1 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A549 cells, Calu-1 cells, MCF-7 cells, NIH/3T3 cells, Neuro-2a cells, PC-3 cells Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse brain tissue Positive IF/ICC detected in: MCF-7 cells Positive FC (Intra) detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information Early growth response 1 (EGR1) is a member of EGR family which are transcriptional factors that contain three repetitive zinc finger DNA binding domains which bind to EGR response elements (ER) to regulate target gene expression. The expression of EGR family members is induced by growth factors, with EGR1 expression being induced by NGF. Increased EGR1 expression activates transcription of other signaling molecules, including CDK5 and tyrosine hydroxylase, and exerts long term effects on neural cell growth and differentiation. Egr-1 binds to the DNA sequence 5'-CGCCCCCGC-3' (Egr-site), thereby activating transcription of target genes whose products are required for mitogenesis and differentiation. Western blotting of nuclear and cytoplasmic fractions of CNS tissue verified the cytoplasmic Egr1 as 110-kDa dimer or after denaturation as a 57-kDa version, which is reflecting its theoretical molecular weight of 57 kDa. In the nucleus Egr1 is found as 75-kDa homo- or heterodimer and as 35-kDa variant under strong denaturation. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: early growth response 1 Calculated Molecular Weight: 58 kDa Observed Molecular Weight: 70-80 kDa GenBank Accession Number: NM_001964 Gene Symbol: EGR1 Gene ID (NCBI): 1958 RRID: AB_2881272 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P18146 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.