Proteintech, 55179-1-AP, ATP1A2-Specific Polyclonal antibody

Size: 20ul / 150ul The ATP1A2-Specific (55179-1-AP) by Proteintech is a Polyclonal antibody targeting ATP1A2-Specific in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 55179-1-AP targets ATP1A2-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: 37°C incubated mouse brain tissue, 37°C incubated rat brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: mouse skeletal muscle tissue, mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information ATP1A2, also named as KIAA0778, belongs to the cation transport ATPase (P-type) family and Type IIC subfamily. It is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. Defects in ATP1A2 are the cause of familial hemiplegic migraine type 2 (FHM2). Defects in ATP1A2 are a cause of alternating hemiplegia of childhood (AHC). This antibody is specific to ATP1A2. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide Calculated Molecular Weight: 112 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: NM_000702 Gene Symbol: ATP1A2 Gene ID (NCBI): 477 RRID: AB_2881284 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P50993 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.