Proteintech, 55205-1-AP, IGFBP5 Polyclonal antibody

Size: 20ul / 150ul The IGFBP5 (55205-1-AP) by Proteintech is a Polyclonal antibody targeting IGFBP5 in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse samples 55205-1-AP targets IGFBP5 in WB, IHC, IF-P, ChIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: SKOV-3 cells, mouse embryo tissue Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information IGFBP5, also named as IBP5, prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. It alter the interaction of IGFs with their cell surface receptors. This antibody is specific to IGFBP5. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat, rabbit Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: insulin-like growth factor binding protein 5 Calculated Molecular Weight: 31 kDa Observed Molecular Weight: 33 kDa GenBank Accession Number: NM_000599 Gene Symbol: IGFBP5 Gene ID (NCBI): 3488 RRID: AB_2736835 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P24593 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.