Proteintech, 55217-1-AP, CYP8B1 Polyclonal antibody

Size: 20ul / 150ul The CYP8B1 (55217-1-AP) by Proteintech is a Polyclonal antibody targeting CYP8B1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 55217-1-AP targets CYP8B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, L02 cells, SMMC-7721 cells Positive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Cytochrome P450 8B1 (CYP8B1) , also named as CP8B, CYP12 and CYPVIIIB1, is an enzyme required for the synthesis of cholic acid (CA) in the classic bile acid synthesis pathway. CYP8B1 controls biliary balance of cholic acid and chenodeoxycholic acid, ultimately regulating the intestinal absorption of dietary lipids. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: cytochrome P450, family 8, subfamily B, polypeptide 1 Calculated Molecular Weight: 58 kDa Observed Molecular Weight: 50-58 kDa GenBank Accession Number: NM_004391 Gene Symbol: CYP8B1 Gene ID (NCBI): 1582 RRID: AB_3670243 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UNU6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.