Proteintech, 55229-1-AP, UPK3A Polyclonal antibody

Size: 20ul / 150ul The UPK3A (55229-1-AP) by Proteintech is a Polyclonal antibody targeting UPK3A in WB, IHC, ELISA applications with reactivity to human, mouse samples 55229-1-AP targets UPK3A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse kidney tissue Positive IHC detected in: mouse bladder tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin III (UPK3A) is a promising new urine marker for bladder cancer detection (PMID: 20346489). Loss of UPK3A expression is associated with clinicopathological features of invasive bladder cancer and poor prognosis in upper tract urothelial carcinoma (PMID: 18313120; PMID: 16686732). UPK3A has a calculated molecular weight of 30.7 kDa, with an apparent molecular weight of 47 kDa due to three potential N-glycosylation sites. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: uroplakin 3A Calculated Molecular Weight: 31 kDa Observed Molecular Weight: 31-33 kDa GenBank Accession Number: NM_006953 Gene Symbol: UPK3A Gene ID (NCBI): 7380 RRID: AB_3670244 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75631 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.