Proteintech, 55234-1-AP, ENO3-specific Polyclonal antibody

Size: 20ul / 150ul The ENO3-specific (55234-1-AP) by Proteintech is a Polyclonal antibody targeting ENO3-specific in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 55234-1-AP targets ENO3-specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, SH-SY5Y cells, HepG2 cells, mouse brain tissue, Jurkat cells Positive IP detected in: HeLa cells Positive IHC detected in: mouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information ENO3, also named as MSE, belongs to the enolase family. It appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13). This antibody is specific to ENO3. It has no cross reaction to ENO1 and ENO2. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: enolase 3 (beta, muscle) Calculated Molecular Weight: 47 kDa Observed Molecular Weight: 42-47 kDa GenBank Accession Number: NM_001976 Gene Symbol: ENO3 Gene ID (NCBI): 2027 RRID: AB_10837363 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P13929 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.