Proteintech, 55251-1-AP, TRPM7 Polyclonal antibody

Size: 20ul / 150ul The TRPM7 (55251-1-AP) by Proteintech is a Polyclonal antibody targeting TRPM7 in WB, ELISA applications with reactivity to human, mouse, rat samples 55251-1-AP targets TRPM7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: SH-SY5Y cells, mouse brain tissue, rat brain tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information Transient receptor potential melastatin 7 (TRPM7), along with the closely related TRPM6, are unique channels that have dual operations: cation permeability and kinase activity. In contrast to the limited tissue distribution of TRPM6, TRPM7 is widely expressed among tissues and is therefore implicated in a variety of cellular functions physiologically and pathophysiologically. The discovery of TRPM7's unique structure imparting dual ion channel and kinase activities shed light onto novel and peculiar biological functions, such as Mg2+ homeostasis, cellular Ca2+ flickering, and even intranuclear transcriptional regulation by a cleaved kinase domain translocated to nuclei. The calculated MW of TRPM7 is 213 kDa, 55251-1-AP can detect a band around 220 kDa. (PMID: 32503408) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: transient receptor potential cation channel, subfamily M, member 7 Calculated Molecular Weight: 213 kDa Observed Molecular Weight: 220 kDa GenBank Accession Number: NM_017672 Gene Symbol: TRPM7 Gene ID (NCBI): 54822 RRID: AB_2935541 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96QT4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.