Proteintech, 55374-1-AP, TRIM33 Polyclonal antibody

Size: 20ul / 150ul The TRIM33 (55374-1-AP) by Proteintech is a Polyclonal antibody targeting TRIM33 in WB, IHC, ELISA applications with reactivity to human samples 55374-1-AP targets TRIM33 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: COLO 320 cells, MCF-7 cells, PC-3 cells Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information TRIM33, also named as KIAA1113, RFG7 and TIF1G, belongs to the TRIM/RBCC family. It acts as an E3 ubiquitin-protein ligase. TRIM33 promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. It may act as a transcriptional repressor. It inhibits the transcriptional response to TGF-beta/BMP signaling cascade. TRIM33 plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3, TRIM33 stimulates erythroid differentiation of hematopoietic stem/progenitor. Defects in TRIM33 are a cause of thyroid papillary carcinoma (TPC). This antibody is specific to TRIM33. The calculated molecular weight of TRIM33 is 122kDa, but the molecular weight of detected is about 140-150 kDa as the same as the result from the paper(PMID:25639486;26592194). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: tripartite motif-containing 33 Calculated Molecular Weight: 122 kDa Observed Molecular Weight: 140-150 kDa GenBank Accession Number: NM_015906 Gene Symbol: TRIM33 Gene ID (NCBI): 51592 RRID: AB_11183035 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UPN9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.