Proteintech, 55375-1-AP, ATP7A-Specific Polyclonal antibody

Size: 20ul / 150ul The ATP7A-Specific (55375-1-AP) by Proteintech is a Polyclonal antibody targeting ATP7A-Specific in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 55375-1-AP targets ATP7A-Specific in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: unboiled mouse lung tissue Positive IP detected in: mouse lung tissue Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information ATPase copper transporting alpha(ATP7A) is a type of transmembrane P-type ATPase for copper transport. ATP7A and ATP7B are homologous genes responsible for copper transport across cell membranes. ATP7A transports copper ions from the Golgi membrane to copper-dependent enzymes (such as dopamine β-hydroxylase) in the secretory pathway. When copper ion concentrations rise, ATP7A relocalizes from the Golgi apparatus to the plasma membrane, promoting copper efflux and thereby protecting cells from copper toxicity. Mutations in ATP7A are associated with Menkes disease (MNK)( PMID: 28389643, 30341172). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ATPase, Cu++ transporting, alpha polypeptide Calculated Molecular Weight: 165 kDa Observed Molecular Weight: 170 kDa GenBank Accession Number: NM_000052 Gene Symbol: ATP7A Gene ID (NCBI): 538 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q04656 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.