Proteintech, 55448-1-AP, B4GALT1 Polyclonal antibody

Size: 20ul / 150ul The B4GALT1 (55448-1-AP) by Proteintech is a Polyclonal antibody targeting B4GALT1 in WB, ELISA applications with reactivity to human, mouse samples 55448-1-AP targets B4GALT1 in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, human placenta tissue, mouse small intestine tissue, mouse testis tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information B4GALT1 is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. B4GALT1 is unique among the beta4GalT genes because it encodes an enzyme that participates both in glycoconjugate and lactose biosynthesis. B4GALT1 has two isoforms with molecular weights of 44 kda and 43 kda that are localized to the cell surface and Golgi apparatus, respectively. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 40-44 kDa GenBank Accession Number: NM_001497 Gene Symbol: B4GALT1 Gene ID (NCBI): 2683 RRID: AB_3670253 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P15291 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.